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KMID : 0387720090200030247
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Korean Journal of Blood Transfusion
2009 Volume.20 No. 3 p.247 ~ p.252
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Chimerism Proven by Flow Cytomety in the Setting of an ABO Discrepancy: A Case Report
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Jeong Eun-Sun
Moon Chang-Jin
Cho Duck
Park Jong-Tae
Ryang Dong-Wook
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Abstract
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Chimerism is an important, yet uncommon cause of ABO phenotype/genotype discrepancies. The propositus was a 28 year-old pregnant women who had an ABO discrepancy, expressing an AweakB RBC phenotype and AB reverse type. Sequencing of ABO exons 6 and 7 revealed a B101/O01 genotype. Neither analysis of 9 short tandem repeats (STR) loci nor HLA typing on DNA extracted from white blood cells demonstrated evidence of chimerism. However, flow cytometric analysis using a PE-conjugated anti-A mouse monoclonal antibody detected a small population (3.8%) of red cells expressing normal A antigen. Based on this, we suggest that flow cytometric analysis is an effective method for the identification of small chimeric populations of RBCs in the immunohematology laboratory. (Korean J Blood Transfus 2009;20:247-252)
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KEYWORD
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Chimera, ABO discrepancy, Flow cytometry
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